Help with sgRNA dose for knockdown

[DrRespectful]

How much sgRNA should I use if Im planning to add sgRNA to WTC11-dCas9-KRAB cells to knock down a gene. The cells already have dCas9..

 

[AverageBrody]

How much sgRNA should I use if Im planning to add sgRNA to WTC11-dCas9-KRAB cells to knock down a gene. The cells already have dCas9..

You should aim for a final concentration of 50-100 nM sgRNA or approximately 100-250 ng per well in a 24well plate, then optimize based on your specific cell density and transfection reagent

 

[DrRespectful]

I see, what is the typical starting cell density per well in a 24-well plate when using this sgRNA concentration range?