CRISPR/Cas9, originally detailed as an immune defense system, has become one of the most widely used gene editing tools for its simplicity of use, ease of design, and capability to target multiple genes. Used it in various systems including plants, microbes and mammalian models. Tho, obstacles like low precision in base editing and off target effects still need to be addressed.
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CRISPR base editing
- Thread starter dexter88
- Start date
Honestly It's incredible how far CRISPR/Cas9 has come, especially considering its origins as a bacterial defense mechanism. But yeah, for all its promise, there are still imperfections like off target edits and imprecise base editing can't be ignored. It's powerful, but still feels like wielding a scalpel with shaky hands.CRISPR/Cas9, originally detailed as an immune defense system, has become one of the most widely used gene editing tools for its simplicity of use, ease of design, and capability to target multiple genes. Used it in various systems including plants, microbes and mammalian models. Tho, obstacles like low precision in base editing and off target effects still need to be addressed.
Crispy Fry
Member
- Joined
- May 23, 2025
- Messages
- 6
CRISPR is the real thing. Clean cuts, easy set-up. Just perfect. But yeah, off-target hits still come ever now and then. Hoping the hi-fi Cas variants will keep improving it then.CRISPR/Cas9, originally detailed as an immune defense system, has become one of the most widely used gene editing tools for its simplicity of use, ease of design, and capability to target multiple genes. Used it in various systems including plants, microbes and mammalian models. Tho, obstacles like low precision in base editing and off target effects still need to be addressed.
GenomeJockey
New member
- Joined
- May 18, 2025
- Messages
- 4
I completely agree, CRISPR has really evolved from just being a defense mechanism in bacteria to becoming a leading tool for gene editing. The precision and simplicity of it are truly remarkable, but those off-target effects definitely make us cautious. Have you had a chance to use any of the hi-fi Cas variants? I'm curious about how much they actually enhance specificity based on your experience